PLEASE, click on the links to the left to distinguish between Core or Olympus equipment!
Olympus FV3000RS confocal microscope $27/hr (G.I. Center members get a supplement).
ACCM Confocal microscope (Leica SP8): $27/hr business hours, $20/hr outside business hours (both wrt fully trained users). Scheduling and billing is through iLab (ACCM Confocal Microscope). ==> ACCM has bought substantial user time on this microscope and will distribute it to departmental researchers based on need and scientific merit, please contact Dr. Mintz(firstname.lastname@example.org) to request a grant of departmental user hours.
Olympus FV1000MP multiphoton microscope: $50/hr.
FISHscope: hourly rate to be determined after the microscope arrives in Spring 2019. We will also have a pass-through single molecule RNA FISH probe set(s) service in collaboration with Image Core Director Prof. Bin Wu's lab. We anticipate our smFISH probe sets will perform as well as commercial RNAscope and Stellaris FISH probes, but costs to JHU research labs will be less, offsetting any rate we charge for FISHscope. Our new FISHscope 'featured app' is smFISH, will be able to be used for other fluorescence experiments.
Other instruments managed by image core: currently $250/lab/year for each instrument used that is not on an hourly rate (that is, not the FV3000RS, SP8 or FV1000MP). We may modify rate for specific instruments to cover service contracts and/or other costs. We may also ask major user labs to help pay for any major repair and/or service contract costs. these instruments include:
* Andor Revolution X1 spinning disk confocal microscope.
* Keyence BZ-X700 "box" microscope (aka imaging plate reader).
* PerkinElmer EnVision plate reader.
* Zeiss inverted microscope (now with Olympus DP80 dual monochrome and color CCD).
** Please note that image core management sometimes has to cancel/postpone user sessions due to required service visits or other reasons. When this happens we will try to make the user's next imaging session be "no charge" (even if longer than the cancelled session).
Please Reserve Your Instrument!!! Ideally 24 or more hours in advance; if not in advance, then right after you fill in the paper sign-in sheet. Reservatons policty is online at
Please read - and follow - our Biosafety Policy
Quick biosafety in image core tips:
* "safety is job #1". If you are not using our equipment in a safe manner, then do not use our equipment.
* do not touch our microscopes,keyboards, computer mice, door knobs, light switches, etc, with lab gloves.
* Yes, handle your SBS plate, imaging dish, other biohazard specimen, with a new lab glove(s) ... after adjusting -- without glove -- the microscope condenser arm, etc, to recieve/remove your specimen.
* Yes, biohazard spills have happened here at JHU/JHMI - see July 2018 tuberculosis spill national news.
* Transport your specimen(s) in a spill resistant tray(s) and/or box(es), i.e. on a lab cart. Do not touch the passenger elevator door buttons, or stairwell handles, with lab gloves.
* our major users include 6 different models of infectious diarrhea, and potentially clinical specimens from G.I., with "C. diff" and/or novel infrectious agents, and Prof. Cindy Sears lab, who have shown that essentially all human colorectal cancers involve contributions from infectious agents (800,000 new cases per year woldwide). It is critical that all users conduct experiments in a manner that is safe for both the user, other users, core staff, custodial staff, and visitors.
* Dr. McNamara is the image core manager and the biosafety officer for the microscope rooms. If you are3 unwilling to follow our biosafety rules, either you and your P.I. should explain to Dr. McNamara and Image Core Director Prof. Bin Wu, how to improve our biosafety policies, or you should not do experiments here.
Plan A: many JHU SOM computers can see our file server. This is the simplest way to transfer files for those with access. Do not give out the name or I.P. address of our server.
Plan B: your MyJH -> your Microsoft OneDrive: Nearly all our PC's can see "The Web" and so can see MyJH. Now (mid-2018) that every JHU staff member gets 5 Terabytes of Microsoft Onedrive "cloud" storage space, you can transfer from the acquisition PC or our server to your OneDrive account. Be sure to logout ("sign off") of your OneDrive session here when you are done -- you need to do this manually. You also need to log out of your MyJH session.
Plan C: You / your lab could 'expose' some "share folder" on your PC or server and transfer from our PC/server to your share. You should only do this with explicit permission of your P.I. and network administrator, since setting up your 'share' incorrectly could expose your PC/server to attack.
NOT Permitted: USB drives are NOT permitted on our computers. We will dispose of them in the Biosafety trash ... which may be full of our host NIH P30DK089502 Center's specialty: infectious diarrhea agents.
Olympus Imaging Suite
July-August 2017: we are considering transitioning to keep this on one microscope/PC
Deep Tissue Scan Capabilities
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