Available services include:
- Advice and consent on sample preparation desired scientific approach
- Some experimental methods and applications include,
- Advanced cell and tissue multicolor imaging using Zeiss LSM-510 META including XYZT, FREP and FRET ... NIH S10 shared instrumentation grant proposal was funded! - Our thanks to the 'confocal' study section membersand staff, the NIH Council-of-Councils, U.S. Congress, and U.S. taxpayers, for funding. Replacement confocal will be an Olympus FV3000RS confocal microscope.
- Leica SP8 inverted confocal microscope - owned by Anesthesiology & Critical Care Medicine (ACCM), managed by us.
- Live cell and tissue imaging using an Olympus FV1000 MP mulitphoton excitation fluorescence NDD4 microscope, with ratiometric ionic probes (Indo-1 or SNARF), CFP/YFP, and "RGB" filter sets.
- Andor Revolution X1 spinning disk confocal microscope with TuCam image splitter and dual EMCCDs.
- Keyence BZ-X700 "box" microscope (no eyepieces), can handle many different specimen formats.
- Measurements of intracellular pH (potentially other ions) on cell populations using PTI’s RatioMaster and QuantMaster spectrofluorometic systems, equipped with temperature controlled perfusion chambers.
- Access to MetaMorph and Volocity software for advanced image analysis, deconvolution, tracking, etc.
- Comprehensive instruction on all facility equipment
- Individualized sample preparation is not available at his time
- Please click on the following link for a complete list of equipment, location and scheduling: Current Equipment
We are not the only light microscopy core facility at Johns Hopkins University. We will try to keep the links below up to date - if you find a non-functional link, or would like us to add a link, please contact George McNamara, firstname.lastname@example.org with information.
|JHU iLabs Organizer||JHU / JHMI wide core resources||
iLabs Organizer ... we suggest you search for confocal
|MicFac||East Baltimore - Physiology Bldg||
lots of equipment and staff
|MPI Core (Neurosci P30)||East Baltimore - PCTB||
multiphoton and confocal
Microscopy and Imaging Core Facility (MICF)
East Baltimore -
Robert H. and Clarice Smith Building
Wilmer Eye Institute
|ICE||East Baltimore - Miller Research Bldg||
Institute for Cellular Engineering
The department has a Nikon epifluorescence microscope equipped with a CCD camera for digital imaging. This unit is a core facility, available to departmental members at large. In addition, the department has an Olympus Fluoview 300, laser-scanning confocal microscope that is shared among several departmental laboratories that competed successfully for an NIH shared instrumentation grant. The confocal microscope is equipped for simultaneous electrophysiological and patch-clamp recording, and has custom lasers permitting CFP/YFP FRET imaging.
MRB Molecular Imaging Service Center and Cancer Functional Imaging Core (MRB - miller Research Bldg)
https://johnshopkins.corefacilities.org/service_center/3787/?tab=equipment (iLab Organizer)
Multiphoton Intravital Microscope
|Cell Imaging Core Facility||The Sidney Kimmel Comprehensive Cancer Center||
https://johnshopkins.corefacilities.org/service_center/3000/?tab=equipment (iLab Organizer)
|Homewood - IIC||
|Carnegie embryology||Carnegie Institute for Science - Department of Embryology (next to JHU Homewood campus)||https://emb.carnegiescience.edu/|
|HI^2||BME & more||
Hopkins Imaging Initiative (BME centric, radiology, image processing, some microscopy)
Welcome to the Hopkins Imaging Initiative (HI^2), a collaborative resource for all of the imaging research at Johns Hopkins University. HI^2 consists of Hopkins researchers who focus on image acquisition, analysis, and application.
Some of our current goals:
Connect researchers who develop and utilize imaging technologies
More cores (for various applications) and links
GCRC "web notes" http://www.hopkinsmedicine.org/webnotes/core_facilities (links to monthly calendar)
Other Baltimore Area Academic Confocal Core's
(note: we and/or the sites may not keep up to date)
|UMd SOM||UMd SOM||