Zeiss Axio Observer.A1 Inverted Microscope (S972) Use iLab
Inverted fluorescent microscope, Olympus DP80 RGB color and NIR capable monochrome camera
Zeiss Axio Observer.A1 Inverted Microscope in Ross S972 has an Olympus DP80 RGB color + monochrome camera, capable of both RGB histology images and fluorescence. Phase contrast is available for several objective lenses.
20191104M (November 4, 2019): we are now on JHU iLab for scheduling.
Ross Imaging Center
If a user needs their PI or administrrtor to set up account numbers (IO#'s in JHU accounting jargon), see
Quck tip, Feb 20, 2019: Optimizing for brightfield image capture, i.e. H&E or IHC (DAB&H):
Big News January 11, 2019: We replaced the DP72 RGB camera with Olympus DP80 RGB + monochrome camera. The two CCD sensors are controlled by the same two CellSens buttons for RGB and monochrome, but now moves a hardware component to switch between the two CCD sensors. This will enable much better imaging of Cy5 and Alexa Fluor 647 and other near infrared (NIR) fluorophores. The DP80 RGB color sensor is identical to the DP72. Please note that currently the eyepieces and DP80 are not parfocal - we recommend spending as much time as possible "live" with the DP80 and little time looking through the eyepieces. We are confident the benefits of having RGB + monochrome CCD sensor options outweight the minor inconvenience of not being parfocal. GM thanks John Gibas (Olympus) and Bill Griffith, (firstname.lastname@example.org), Sr. Instrument Designer, BME Machine shop, Traylor 4th floor, for big help.
Jan 14, 2019 note: we will probably remove the Eppendorf micromanipulator from this microscope for lack of use. It will remain close by and can be set up again. Users interested in it will need to plan ahead and contact George to see about getting it set up on the "A1".
Suitable specimens include:
* microscope slides ... histology and/or fluorescence (DAPI, green, red, the microscope has a Cy5 filter set - please use the DP80's monochrome camera for single channel fluorescence.
* multiwell plates.
* 35 mm or 60 mm plastic (modest resolution and fluorescence sensitivity) and glass bottom (i.e. Mattek.com P35 series imaging dishes).
Our CellSens 2.02 license includes:
MIA = Multi Image Alignment Algorithm ... panorama
EFI = Extended Focal Imaging
for summary, see brochure at https://unicam.hu/files/olympus-cellsens/cellSens_Attekintes.pdf (PDF also on our server and on acquisition PC).
Olympus CellSens tutorials (videos) online at https://www.olympus-lifescience.com/en/software/cellsens/?utm_content=sf92551011&utm_medium=social-media&utm_source=googleplus&utm_campaign=Olympus%20Life%20Science&sf92551011=1#!cms[tab]=%2Fsoftware%2Fcellsens%2Fresources
McNamara 20190517Fri – Zeiss AxioObserver A1 filter sets
Zeiss ## filter set specifications are from https://www.micro-shop.zeiss.com/en/de/shop/filterAssistant/filtersets
|B||49||G 365||FT 395||BP 445/50||488049-9901-000|
|G||38||BP 470/40||FT 495||BP 525/50||000000-1031-346|
|R||43||BP 545/25||FT 570||BP 605/70||000000-1114-101|
TBP 400 + 495 + 570
|TFT 410 + 505 + 585||TBP 460 + 530 + 625||488025-0000-000|
Our Olympus DP80 camera has dual RGB and monochrome CCD sensors (Cellsens acquisition has a toggle for RGB and monochrome). The monochrome sensor is much higher sensitivity, including for Cy5, so should be used for fluorescence. That makes the “RGB” filter set only useful for you to look by eye. The DP80 RGB sensor is ideal for H&E, H&DAB, and similar transmitted light applications (empty cube position between Cy5 and DAPI cubes, or RRGB cube – your choice).