Contact Us

Ross Fluorescence Imaging Core
Johns Hopkins University
School of Medicine
720 Rutland Avenue
913S Ross Research Bldg
Baltimore,  MD 21205-2195
Phone 410-614-0134

Cell 305-764-2081 George McNamara gmcnamara@jhmi.edu 

 

Director
Prof. Bin Wu

bwu20@jhmi.edu

Associate Director
Prof. Brian O’Rourke
bor@jhmi.edu
(410) 614-0034

Manager

George McNamara, PhD

Image Core Manager, Ross Bldg 913S (Service Corridor)

Ross Fluorescence Imaging Center

http://confocal.jhu.edu

Johns Hopkins University School of Medicine

gmcnama2@jhmi.edu

410-614-0134 office

 


 

Olympus Imaging Center (hosted by our core, Ross Bldg S910)

John Gibas - Olympus America

Olympus Microscope and Imaging Sales Rep, Mid-Atlantic Region ( Greater Baltimore)
Scientific Solutions Group, Olympus America Inc.

john.gibas@olympus-ossa.com

 

Jason Brenner
Confocal and Multi Photon Sales Representative
Olympus America Incorporated 
jason.brenner@olympus-ossa.com

 

 


 

Acknowledging our image core

Please include our NIH 5P30DK089502 center grant number in the Acknowledgements or Funding Sources section of your manuscripts.

For example:

 

    " ... P30DK089502 (to Hopkins Digestive Diseases Basic and Translational Core Center) ...".

Please send us all your publications that use and acknowledge our imaging core! Ideally an email with the reference(s) in text and PDF attachment.

 

 

Here is a narrative (now somewhat out of date) about our core:

5P30DK089502-07

P30 grant Core B-Imaging - Olga Kovbasnjuk, Hopkins Digestive Diseases Basic and Translational Core Center (DDBTRCC)

http://grantome.com/grant/NIH/P30-DK089502-07-7316

 Abstract
The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.

Public Health Relevance
Imaging Core B: Narrative The DDBTRCC Imaging Core B is designed to provide our Research Base investigators equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles.

*****

 

The Leica SP8 confocal microscope is owned by Anesthesiology and Critical Care Medicine (ACCM) and managed by us. If your project(s) on the Leica SP8 are "pretty much self sufficient" please acknowledge ACCM. If you obtained substantial help from our core (ex. from George McNamara), please acknowledge both ACCM and our P30 grant number.

 

Equipment

Keyence BZ-X700 with Tokai Hit Incubator

Keyence BZ-X700 with Tokai Hit Incubator. 20170808: Sato-San from Tokai Hit installed a new incubator lid (a user broke a cable...

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Leica SP8 (iLab: ACCM Confocal Microscope)

The Leica SP8 confocal microscope is on a Leica DMi8 inverted microscope stand (DMi8CEL).  June 1, 2018 was the start of...

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Andor Revolution X1 Spinning Disk confocal inverted...

Andor Revolution X1 Spinning Disk confocal inverted microscope. * Andor 4 laser stack (405, 488, 561, 640 nm). * Olympus...

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Zeiss Axio Observer.A1 Inverted Microscope (S972)

Zeiss Axio Observer.A1 Inverted Microscope in Ross S972 has an Olympus DP72 RGB color camera, capable of both RGB histology...

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