Ross Fluorescence Imaging Core
Johns Hopkins University
School of Medicine
720 Rutland Avenue
913S Ross Research Bldg
Baltimore, MD 21205-2195
Cell 305-764-2081 George McNamara firstname.lastname@example.org
Prof. Bin Wu
Prof. Brian O’Rourke
George McNamara, PhD
Image Core Manager, Ross Bldg 913S (Service Corridor)
Ross Fluorescence Imaging Center
Johns Hopkins University School of Medicine
Olympus Imaging Center (hosted by our core, Ross Bldg S910)
John Gibas - Olympus America
Olympus Microscope and Imaging Sales Rep, Mid-Atlantic Region ( Greater Baltimore)
Scientific Solutions Group, Olympus America Inc.
Confocal and Multi Photon Sales Representative
Olympus America Incorporated
Acknowledging our image core
Please include our NIH 5P30DK089502 center grant number in the Acknowledgements or Funding Sources section of your manuscripts.
" ... P30DK089502 (to Hopkins Digestive Diseases Basic and Translational Core Center) ...".
Please send us all your publications that use and acknowledge our imaging core! Ideally an email with the reference(s) in text and PDF attachment.
Here is a narrative (now somewhat out of date) about our core:
P30 grant Core B-Imaging - Olga Kovbasnjuk, Hopkins Digestive Diseases Basic and Translational Core Center (DDBTRCC)
The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.
Public Health Relevance
Imaging Core B: Narrative The DDBTRCC Imaging Core B is designed to provide our Research Base investigators equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles.
The Leica SP8 confocal microscope is owned by Anesthesiology and Critical Care Medicine (ACCM) and managed by us. If your project(s) on the Leica SP8 are "pretty much self sufficient" please acknowledge ACCM. If you obtained substantial help from our core (ex. from George McNamara), please acknowledge both ACCM and our P30 grant number.