Keyence BZ-X700 use iLab
Location: Ross 9th floor
Keyence BZ-X700 with Tokai Hit Incubator
20191104M (November 4, 2019): we are now on JHU iLab for scheduling.
Ross Imaging Center
If a user needs their PI or administrator to set up account numbers (IO#'s in JHU accounting jargon), see
** Please note that image core management sometimes has to cancel/postpone user sessions due to required service visits or other reasons. When this happens we will try to make the user's next imaging session be "no charge" (even if longer than the cancelled session).
20190711 update (really reminder to GM about priority vs non-priority users: Donowitz and Andersen groups can schedule Keyence asfare in advance as they like. Other groups reservagtions awre valid only if <1 week in advance.
20190625 Keyence Usage Policy Update:
the Policy is NOW being enforced for only members of specific labs can make reservations more than 7 days in advance
The Keyence microscope is owned by two labs and managed by the image core. The two labs -- Prof. Mark Donowitz (G.I.) and Prof. Peter Andersen (Cardiology, with Prof. Chulan Kwan) have priority access. As of now, this means members of these two labs can make reservations 1 week in advance of their session start. All other users should reserve less than one week in advance -- and shouldbe of "limited time", i.e. maximum 3 days. No one should make reservations for other.
Only employees in the labs of these P.I.'s have priority access, and only for their experiments::
==> multiple fields of view --> stitch ... alternative: our Zeiss Axio Observer.A1 Inverted Microscope now has "manual MIA" stitching in Olympus CellSens. I note that both Adobe Photoshop CS__ and the free Microsoft ICE (Image Composite Editor) can stitch. Fiji ImageJ also has various stitchers.
Keyence Access Policy (20180914 update) -- As of 20190625 will be strictly enforced.
- The Keyence microscope is owned by two labs and managed by the image core. The two labs -- Prof. Mark Donowitz (G.I.) and Prof. Peter Andersen (Cardiology) have priority access. As of now, this means members of these two labs can make reservations 1 week in advance of their session start. All other users should reserve less than one week in advance --- and be of limited duration.
- Multi-hour experiments: should be conducted overnight, not take up significant workday hours (ex. 4pm Tue -> 9am Wed)..
- Multi-day experiments: should be conducted over weekend (ex. 4pm Fri -> 9am Mon).
20181018 update: Ryan Parent is primary Keyence contact (Ari Klein moved to Florida) and Takanori Tsuchiya is primary Tokai Hit contact (Sato-San returned to Japan).
Life Science Microscope Team
Keyence Corporation of America
700 American Ave Suite 100
King of Prussia, PA 19406
20170808: Sato-San from Tokai Hit installed a new incubator lid (a user broke a cable of the previous lid). All users should use the Tokai Hit incubator inserts when using a microscope slide or 35 mm dish (holds 2 dishes, if using only one, put an empty dish on other opening to keep the humid air in the incubator volume, not into the Keyence body) or 50 or 60 mm dish holder (please contact George if you want to use the 100 mm dish holder that came with the Keyence BZ-X700 microscope).
* SBS plates
* microscope slide
* one or two 35 mm plastic dishes or imaging dishes the high NA objectives require use of imaging dish). *** if using one dish with humidified air, cover the other opening with a dish.
* 50 or 60 mm plastic or imaging dish (this holder can also accept a standard 75x25 mm microscope slide).
My special thanks to Sato-San of Tokai Hit for excellent support and training.
Glass bottom dishes and SBS plates can be purchased from Mattek, https://www.mattek.com/store-category/cultureware/glass-bottom-dishes/
Most users should order "1.5" thickness coverglasses (specification refers to manufactururing in 160 to 190 um range); the "0.170" millimeter thickness = 170 um are higher precision. Several other vendors also offer imaging dishes and with a dentist circular saw and silicone cement (a.k.a. aquarium glue sealer), I used to make my own (and still have some 40x50 mm coverglass in 100 mm diameter petri dishes).
Nice information on coverglasses:
Size Thickness (inches) Thickness (mm)
#00 0.0020-0.0033 0.05-.008
#0 0.0033-0.0051 0.08-0.13
#1 0.0051-0.0063 0.13-0.16
#1 1/2 0.0063-0.0075 0.16-0.19
#2 0.0075-0.0098 0.19-0.25
#3 0.0098-0.0138 0.25-0.35
#4 0.0170-0.0250 0.43-0.64
I note that Hausser Scientific [without glass in name] offers 0.4 mm and 0.5 mm thick coverglasses; I included 1.0 mm, which is standard microscope slide thickness:
Catalog # Description
1461 20mm x 26mm x 0.4mm
5000 20mm x 26mm x 0.5mm
5010 25mm x 28mm x 0.5mm
5020 25mm x 33mm x 0.5mm
5030 27mm x 37mm x 0.5mm
5040 Sedgewick Rafter cover glass 25mm x 60mm x 0.5mm
5051 Petroff-Hausser 20mm x 26mm x 0.2mm
5060 Whipple eyepiece micrometer 21mm dia.
5075 Graduated Howard Mold Cover Glass, Unlaminated
5075L Graduated Howard Mold Cover Glass, Laminated
5080 Howard thin 28mm x 33mm x 0.5mm
5090 Howard thick 28mm x 33mm x 1.0mm
5400 Nageotte 30mm x 33mm x 0.5mm
5411 Nageotte 30mm x 33mm x .17mm (1 oz box) disposable