Keyence BZ-X700 use Google Calendar

Keyence BZ-X700
Location: Ross 9th floor

Keyence BZ-X700 with Tokai Hit Incubator

20190705Fri: we now use Google Calendar

https://calendar.google.com/calendar?cid=bjJiM2FyNnUxcGdmMHRvZHRqaW1ybzg5MXNAZ3JvdXAuY2FsZW5kYXIuZ29vZ2xlLmNvbQ

(we have a meeting in early August 2019 to discuss whether to move to JHU iLab).

20190625 Keyence Usage Policy Update: We are now enforcing our 20180914 Policy

20190711 update (really reminder to GM about priority vs non-priority users: Donowitz and Andersen groups can schedule Keyence asfare in advance as they like. Other groups reservagtions awre valid only if <1 week in advance.

20190625 Keyence Usage Policy Update: 

the Policy is NOW being enforced for only members of specific labs can make reservations more than 7 days in advance

The Keyence microscope is owned by two labs and managed by the image core. The two labs -- Prof. Mark Donowitz (G.I.) and Prof. Peter Andersen (Cardiology, with Prof. Chulan Kwan) have priority access. As of now, this means members of these two labs can make reservations 1 week in advance of their session start. All other users should reserve less than one week in advance -- and shouldbe of "limited time", i.e. maximum 3 days. No one should make reservations for other.

 

  • Multi-hour timelapse experiments: should be conducted overnight, not take up significant workday hours (ex. 4pm Tue -> 9am Wed, not take up all day).  Non timelapse experiments, i.e. "lots of slides", should be conducted to be as time efficient as possible. 
  • Multi-day experiments: should be conducted over weekend (ex. 4pm Fri -> 9am Mon).
  • Very long multi-day experiments, i.e. 9 days, are permitted for thesespecific labs.

Only employees in the labs of these P.I.'s have priority access, and only for their experiments::

  • Prof. Mark Donowitz and junior faculty in Mark's group.
  • Prof. Nick Zachos
  • Prof. Jennifer Foulke-Abel
  • Professors Peter Anderson and Chulan Kwan.

Potential alternatives:

  • Olympus FV3000RS confocal microscope ... intended mostly for daytime imaging. There is one project in the NIH S10 grant with multi-day timelapse sessions written in, so this project has high priority access (do need to schedule in advance). Other projects should aim to use FV3000RS nights and weekends, as much as possible (avoiding multiple week nights).
  • Andor Revolution X1 ... intended for "daytime" fast live cell imaging. Capable of 37 C incubator experiments, but priority is live cell imaging -- mostly by Prof. Brian O'Rourke's lab and cardiology collaborators. Please check with Prof. O'Rourke and George McNamara about upcoming usage - the daytime fast live imaging has priority.
  • We note that there is an Essen Biosciences Incucyte S3 imager in Ross Bldg. The Incucyte can image brightfield/phase contrast, green and red fluorescence, and hold up to 6 SBS plates or T25, T75 flasks. Please contact George McNamara for the name of the P.I. of that imager to determine if you could conduct experiments on their instrument (biosafety issues and/or heavy use may cause them to have to decline use to you). 

.

==> multiple fields of view --> stitch ... alternative: our Zeiss Axio Observer.A1 Inverted Microscope now has "manual MIA"  stitching in Olympus CellSens. I note that both Adobe Photoshop CS__ and the free Microsoft ICE (Image Composite Editor) can stitch. Fiji ImageJ also has various stitchers.

Keyence Access Policy (20180914 update) -- As of 20190625 will be strictly enforced.

  • The Keyence microscope is owned by two labs and managed by the image core. The two labs -- Prof. Mark Donowitz (G.I.) and Prof. Peter Andersen (Cardiology) have priority access. As of now, this means members of these two labs can make reservations 1 week in advance of their session start. All other users should reserve less than one week in advance --- and be of limited duration.
  • Multi-hour experiments: should be conducted overnight, not take up significant workday hours (ex. 4pm Tue -> 9am Wed)..
  • Multi-day experiments: should be conducted over weekend (ex. 4pm Fri -> 9am Mon).

20181018 update: Ryan Parent is primary Keyence contact (Ari Klein moved to Florida) and Takanori Tsuchiya is primary Tokai Hit contact (Sato-San returned to Japan).

Ryan Parent

RParent@keyence.com

Sales Engineer

Life Science Microscope Team

Keyence Corporation of America

700 American Ave Suite 100

King of Prussia, PA 19406

Takanori Tsuchiya 
Mail: t-tsuchiya@tokaihit.com 
URL: http://www.tokaihit.com/english/top 
TOKAI HIT USA INC. 
Two Bala Plaza Suite 300, Bala Cynwyd, PA 19004 
Tel: +1 610 660 7784 
(株)東海ヒット Tokai Hit Co., Ltd. 
静岡県富士宮市源道寺町306-1, 418-0074 
Tel: +81 544 24 6699 

20170808: Sato-San from Tokai Hit installed a new incubator lid (a user broke a cable of the previous lid). All users should use the Tokai Hit incubator inserts when using a microscope slide or 35 mm dish (holds 2 dishes, if using only one, put an empty dish on other opening to keep the humid air in the incubator volume, not into the Keyence body) or 50 or 60 mm dish holder (please contact George if you want to use the 100 mm dish holder that came with the Keyence BZ-X700 microscope).

* SBS plates

* microscope slide

* one or two 35 mm plastic dishes or imaging dishes the high NA objectives require use of imaging dish). *** if using one dish with humidified air, cover the other opening with a dish.

* 50 or 60 mm plastic or imaging dish (this holder can also accept a standard 75x25 mm microscope slide).

My special thanks to Sato-San of Tokai Hit for excellent support and training. 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Glass bottom dishes and SBS plates can be purchased from Mattek, https://www.mattek.com/store-category/cultureware/glass-bottom-dishes/

Most users should order "1.5" thickness coverglasses (specification refers to manufactururing in 160 to 190 um range); the "0.170" millimeter thickness = 170 um are higher precision. Several other vendors also offer imaging dishes and with a dentist circular saw and silicone cement (a.k.a. aquarium glue sealer), I used to make my own (and still have some 40x50 mm coverglass in 100 mm diameter petri dishes). 

Nice information on coverglasses:

http://www.hausserscientificglass.com/products/precision-glass.html

Size     Thickness (inches)   Thickness (mm)
#00        0.0020-0.0033           0.05-.008
#0          0.0033-0.0051           0.08-0.13
#1          0.0051-0.0063           0.13-0.16
#1 1/2    0.0063-0.0075           0.16-0.19
#2          0.0075-0.0098           0.19-0.25
#3          0.0098-0.0138           0.25-0.35
#4          0.0170-0.0250           0.43-0.64

I note that Hausser Scientific [without glass in name] offers 0.4 mm and 0.5 mm thick coverglasses; I included 1.0 mm, which is standard microscope slide thickness:

http://www.hausserscientific.com/products/cover_glasses_slips.html

Catalog #       Description
1461               20mm x 26mm x 0.4mm
5000               20mm x 26mm x 0.5mm
5010               25mm x 28mm x 0.5mm
5020               25mm x 33mm x 0.5mm
5030               27mm x 37mm x 0.5mm
5040               Sedgewick Rafter cover glass 25mm x 60mm x 0.5mm
5051               Petroff-Hausser 20mm x 26mm x 0.2mm
5060               Whipple eyepiece micrometer 21mm dia.
5075               Graduated Howard Mold Cover Glass, Unlaminated
5075L             Graduated Howard Mold Cover Glass, Laminated
5080               Howard thin 28mm x 33mm x 0.5mm
5090               Howard thick 28mm x 33mm x 1.0mm
5400               Nageotte 30mm x 33mm x 0.5mm
5411               Nageotte 30mm x 33mm x .17mm (1 oz box) disposable


Reserve Equipment

Categories