WELCOME

 

The Ross Fluorescence Imaging Core site is under new management (May 2017). John Gibas is now with Olympus. George McNamara is now the image core manager. Prof. Olga Kovbasnjuk has moved to University of New Mexico. Prof. Bin Wu is now the image core director. 

There will be more content and changes coming to these pages in the near future.  All users should provide JHU IO Numbers (account numbers) for billing. We will also be slowly transitioning from the sign up sheets that we have been using to the online scheduler

 Please come and see me to verify that you are registered in the new database, all your current infomation is up to date and you can actually login to use the site.  Sorry for the delay and thanks for your patience.

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October 30, 2019 news

1. Ross Imaging Center (may) go live on iLab on Monday Nov 4, 2019, expected URL is

iLab      https://johnshopkins.corefacilities.org/equipment/447115 

 Trained users will need a valid JHU account (aka IO#) to make reservations. New users should contact Dr. George McNamara, core manager, about training. For the Fv3000RS and SP8 confocal microscope you can expect to be charged $127/hr for four hours (usually two 2 hour sessions -- assumes you have research level light microscopy experience and need training with respect to our confocal microscopes). This is $127 = $100 trainer's time + $27/hr instrument time. We do not do classroom training - you can get that through Prof. Scot Kuo, MicFac, https://microscopy.jhmi.edu/index.htm ... if you have little or no knowledge of light microscopy, you should tell us in advance, and be prepared t pay for additional time to get training (10 hours at $127/hr may or not be enough). You can also consider starting on one (or more) of our basic microscopes and use that.

  Please note that the Leica SP8 confocal microscope is in the ACCM Confocal Microscope Core (we manage the SP8 for ACCM).

Our Conte Center web site has been updated

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September 18, 2019 news

Schedulers are moving to Agilent iLab Organizer ("iLab")  in October 2019 ("go live" expected Mon Nov 4, 2019). We have been using Google Calendars for a couple of months since this web site's scheduler hosting had issues.

Data transfer: 

1. Please "save locally" (on core PC's that have data drives -- do not save any data on any of our C: drives and NEVER use a USB stick or portable drive on any image core PC ... these are subject to confiscation and being tossed in the biosafety trash) and , and upload to your MyJHU -> Cloud -> Microsoft OneDrive.

Acces to our file server:

==> Note: please do not publicize the name of our file server. 

If you have a compelling reason to use use our file server, and lose access because your computer is now on a JHU SOM domain, you may be able to connect using Windows Map Network Drive, by selecting "Connect using different credentials" and appropriate server name and path ... you may need an account on our server (hint: use JHU's Microsoft OneDrive instead, please).

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August 6, 2019 news:

  • FISHscope now operational (ok, not quite: we had a loaner Lumencor SPECTRA X from Olympus, that demo period ended, we are waiting for our Lumencor SPECTRA III-360 to arrive) ... currently restricted use to the single molecule RNA FISH the microscope was purchased for.
  • Andor Revolution X1 spinning disk confocal microscope - now Legacy instrument - operational for existing fully trained users, if laser(s) etc fail, not planning on replacing components. Has MetaMorph Premier, so can be used as an additional offline workstation (few users use Pemier features).
  • Image Core usage: FV3000RS, ACCM Leica SP8, Keyence BZ-X710 "all-in-one" microoscopes very heavy use.

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June 1, 2018 the ACCM Leica SP8 confocal microscope we manage is on iLab

https://johnshopkins.corefacilities.org/service_center/3804/

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July 20, 2018: Zeiss LSM510META confocal microscope has been retired.

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August 3, 2018 update:

  • Olympus FV3000RS confocal microscope has been funded by NIH S10 grant proposal. we expect to have 'up and using' by end of August 2018.
  • FISHscope expected to be funded (NIH P30 G.I. Center supplement plus matching funds from us) (really: smFISHscope). Microscope delivery asnticipated "autumn 2018".
  • G.I. single molecule fluorescence in situ hybridization (smFISH) first 30 G.I. centric probe sets development and implementation funded.

Instructions to (hopefully) see our file server is now on our McTips page

http://confocal.jhu.edu/mctips 

(GM can also send as a Word doc).

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August, 2018: Olympus FV3000RS confocal microscope is here! 

November 2018: The NIR unit arrived, now have:

7 laser lines: 405, 445, 488, 514, 561,640, 730 nm.

6 detectors:

  * 4 GaAsP (internal, spectral bandpasses, 400-800nm)

  * 2 GaAs (external, NIR1: 690-750nm, NIR2: 780LP; conventional IX83 filter cube, so could buy additional cubes for specialty needs). We would love a contribution to get additional external detectors!

--> corssover point of sensitivity of GaAsP (better vis) and GaAs (better NIR) is ~712 nm. 

March 2019: FV3000RS ZDC 830 nm installed (previously was shorter wavelength). ZDC = Zero Drift Compensation.

April 22, 2019: FISHscope has been ordered. Main use will be single molecule RNA fluorescence in situ hybridization (smFISH). 

  * Olympus IX83 inverted microscope ('gliding' stage, motorized Z, 2nd deck is Sutter 10-3 emission filter wheel).

  * Lumencor SPECTRA 3-360 lamp (8 LEDs, 360 nm to 750 nm).

  * Semrock Penta filter cube (penta exciter, penta dichroic,penta emission in cube) and emission filters for Sutter wheel.

  * Hamamatus ORCA-FLASH4.0LT.

  * LCI optogenetic unit for screening.

  * Generously funded by our NIH P30 G.I. Conte Center grant supplement and internal funds.

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gmcnama2@jhmi.edu

geomcnamara@earthlink.net

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