WELCOME

 

The Ross Fluorescence Imaging Core site is under new management (May 2017). John Gibas is now with Olympus. George McNamara is now the image core manager. Prof. Olga Kovbasnjuk has moved to University of New Mexico. Prof. Bin Wu is now the image core director. 

There will be more content and changes coming to these pages in the near future.  All users should provide JHU IO Numbers (account numbers) for billing. We will also be slowly transitioning from the sign up sheets that we have been using to the online scheduler

 Please come and see me to verify that you are registered in the new database, all your current infomation is up to date and you can actually login to use the site.  Sorry for the delay and thanks for your patience.

//

June 1, 2018 the ACCM Leica SP8 confocal microscope we manage is on iLab

https://johnshopkins.corefacilities.org/service_center/3804/

//

July 20, 2018: Zeiss LSM510META confocal microscope has been retired.

//

August 3, 2018 update:

  • Olympus FV3000RS confocal microscope has been funded by NIH S10 grant proposal. we expect to have 'up and using' by end of August 2018.
  • FISHscope expected to be funded (NIH P30 G.I. Center supplement plus matching funds from us) (really: smFISHscope). Microscope delivery asnticipated "autumn 2018".
  • G.I. single molecule fluorescence in situ hybridization (smFISH) first 30 G.I. centric probe sets development and implementation funded.

Instructions to (hopefully) see our file server is now on our McTips page

http://confocal.jhu.edu/mctips 

(GM can also send as a Word doc).

//

August, 2018: Olympus FV3000RS confocal microscope is here! 

November 2018: The NIR unit arrived, now have:

7 laser lines: 405, 445, 488, 514, 561,640, 730 nm.

6 detectors:

  * 4 GaAsP (internal, spectral bandpasses, 400-800nm)

  * 2 GaAs (external, NIR1: 690-750nm, NIR2: 780LP; conventional IX83 filter cube, so could buy additional cubes for specialty needs). We would love a contribution to get additional external detectors!

--> corssover point of sensitivity of GaAsP (better vis) and GaAs (better NIR) is ~712 nm. 

March 2019: FV3000RS ZDC 830 nm installed (previously was shorter wavelength). ZDC = Zero Drift Compensation.

April 22, 2019: FISHscope has been ordered. Main use will be single molecule RNA fluorescence in situ hybridization (smFISH). 

  * Olympus IX83 inverted microscope ('gliding' stage, motorized Z, 2nd deck is Sutter 10-3 emission filter wheel).

  * Lumencor SPECTRA 3-360 lamp (8 LEDs, 360 nm to 750 nm).

  * Semrock Penta filter cube (penta exciter, penta dichroic,penta emission in cube) and emission filters for Sutter wheel.

  * Hamamatus ORCA-FLASH4.0LT.

  * LCI optogenetic unit for screening.

  * Generously funded by our NIH P30 G.I. Conte Center grant supplement and internal funds.

//

gmcnama2@jhmi.edu

geomcnamara@earthlink.net

Hopkins Equipment

MetaMorph Key 2 (#4646) (AxioImager Upright license)

July-August 2017: we are considering transitioning to keep this license to be kepy full time on the Zeiss AxioImager /...

Read More

MetaMorph Key 1 (#34135)

MetaMorph Key 1 is license #34135. Ross 9th floor users: If your preferred Windows PC does not have this license installed,...

Read More

zzzZeiss Axio Imager Upright Microscope, optionally...

7/2017: The Zeiss Axio Imager.M2 upright fluorescence microscope is in Ross S972 (S=service corridor) with Photometrics...

Read More

Olympus FV1000MP Multiphoton Excitation Fluorescence...

Olympus FV1000MP Multiphoton Excitation Fluorescence Microscope (MPEF)   *** News 20170901: Our FV1000MP does not...

Read More

Andor Revolution X1 Spinning Disk confocal inverted...

** Room lights policy: Ross S910 overhead lights are controlled on "first come, first served" basis. That is, the first user to...

Read More

Olympus Equipment

No results for Olympus Equipment