Olympus FV1000MP Multiphoton Excitation Fluorescence Microscope (MPEF) USER Google Calendar
Deep Tissue Scan Capabilities
Olympus FV1000MP Multiphoton Excitation Fluorescence Microscope (MPEF)
News 20190709 (July 9, 2019) Now uses Google Calendar for reservations
Our FV1000MP does not currently have this capability (hint: a modest financial contribution from you might be able to add it; or please help us purchase a modern MPEF microscope with MP-FAIM capability, such as Olympus FV-MP) -- Ralph Weissleder et al published a terrific article on using multiphoton excitation fluorescence anisotropy (MP-FAIM) to image and quantify fluorescent conjugated drug binding specifically to the target protein ("receptor"). In this paper they used PARPi-BODIPY, with the unlabeled PARPi as a competitive inhibitor to block binding (and lots of other controls). They have separately published BRAF(V600E)i-BODIPY (Mikula et al 2017) and other fluorescent drug ligands.
Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging.
Vinegoni C, Fumene Feruglio P, Brand C, Lee S, Nibbs AE, Stapleton S, Shah S, Gryczynski I, Reiner T, Mazitschek R, Weissleder R.
Nat Protoc. 2017 Jul;12(7):1472-1497. doi: 10.1038/nprot.2017.043.
20190626Wed: here are our four filter cubes ... each is "double decker". In principle, we could buy additional empty cubes, plus appropriate beamsplitters and emission filters, for more fluorophores (new filters would likely have better performance because not 10 years old). In practice, no one has needed new filter sets.