Olympus FV1000MP Multiphoton Excitation Fluorescence Microscope (MPEF)

Deep Tissue Scan Capabilities
Location: S972

Olympus FV1000MP Multiphoton Excitation Fluorescence Microscope (MPEF)

 

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News 20170901:

Our FV1000MP does not currently have this capability (hint: a modest financial contribution from you might be able to add it; or please help us purchase a modern MPEF microscope with MP-FAIM capability, such as Olympus FV-MP) -- Ralph Weissleder et al published a terrific article on using multiphoton excitation fluorescence anisotropy (MP-FAIM) to image and quantify fluorescent conjugated drug binding specifically to the target protein ("receptor"). In this paper they used PARPi-BODIPY, with the unlabeled PARPi as a competitive inhibitor to block binding (and lots of other controls). They have separately published BRAF(V600E)i-BODIPY (Mikula et al 2017) and other fluorescent drug ligands.

Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging.
Vinegoni C, Fumene Feruglio P, Brand C, Lee S, Nibbs AE, Stapleton S, Shah S, Gryczynski I, Reiner T, Mazitschek R, Weissleder R.
Nat Protoc. 2017 Jul;12(7):1472-1497. doi: 10.1038/nprot.2017.043. 
PMID: 28686582
http://www.nature.com/nprot/journal/v12/n7/full/nprot.2017.043.html


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