Zeiss LSM 510META confocal optics on AxioObserver.Z1 inverted microscope

Standard fluorescent point scanning confocal with one spectral detector
Location: 913

Zeiss LSM 510META confocal optics on an automated AxioObserver.Z1 inverted microscope.

Laser lines: 405, 458, 476, 488, 514, 561, 633 nm.

Detectors: ChS (S for Spectral; please note only 550-780 nm 'bands' functional), Ch1 (commonly used for DAPI, Hoechst, and 458nm IRM), Ch2 (commonly used for "green", Alexa Fluor 488, GFP).

* has some processing and visualization software modules.

 

Please note: The LSM510 is obsolete in that Zeiss has stopped offering service contracts. Zeiss has also avoided sending us formal quotes, or even informal estimates, on the several components that have failed since a service visit late 2015. We hope to not spend any additional money on this obsolete instrument. Its main value is the scanhead can be traded in for credit toward a new confocal microscope (we submitted in 5/2017 a shared instrumentation grant to NIH for an Olympus FV3000RS confocal inverted microscope).

Known issues as of 8/2017:

* lack of parfocality between eyepieces and LSM light paths. Usually eyepieces are ~80 um higher, so "if you can see in focus by eye, the confocal will usually be able to acquire".

* ChS spectral detector is half dead in that the "left" half detectors modules no longer "see" light". The Zeiss service engineer speculates that the prism or other 'META' component that directs light from ~380-~550 nm, failed. They also informed us that a repair would involve shipping the entire confocal microscope (possibly including the microscope stand, hopefully not also the laser cart) back to Germany to get this serviced. This is not economically feasible for such an obsolete instrument (plus: not under service contract, so if the right half "failed" the day after return, its repair would need another round trip.).

 

George wishes to thank John Gibas (predeccessor, now at the Olympus Imaging Center hosted by our JHU Center) and Baltimore Precision Instruments (BPI, http://www.baltpi.com ), for identifying and purchasing -- at a very nice price -- for our Center the excellent Zeiss 40x/1.4NA oil immersion objective lens. This is the "workhorse" objective lens for this microscope, and BPI identified and sold us this as a "used" lens at an excellent price. BPI also did an outstanding job with respect to service of the metal halide lamp "fluorescence-by-eye" illuminator for this microscope.


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